Tryptose Phosphate Broth (TPB) for Fastidious Bacteria

Tryptose Phosphate Broth (TPB) is a dehydrated liquid culture medium formulated for the cultivation and enrichment of fastidious bacteria.
It provides a balanced nutrient environment suitable for organisms with higher nutritional requirements and is widely used in microbiology laboratories for routine cultivation and research applications.

The medium can also be prepared as a semi-solid medium by adding agar when required, offering flexibility for different laboratory protocols.

🔬 Product Applications

• Cultivation of fastidious microorganisms

• Enrichment culture in microbiological testing

• Routine laboratory microbiology

• Research and quality control applications

📦 Product Specifications

• Product Name: Tryptose Phosphate Broth

• English Name: Tryptose Phosphate Broth

• Form: Dehydrated powder

• Package Size: 250 g / bottle

• Storage Condition: Room temperature, dry and sealed

• Shelf Life: 3 years

🧾 Medium Composition (per liter)

• Tryptose – 20.0 g

• Dextrose – 2.0 g

• Disodium Phosphate – 2.5 g

• Sodium Chloride – 5.0 g

Final pH: 7.3 ± 0.2 (25 °C)

⚙️ Directions for Use

1. Suspend 29.5 g of the medium in 1 liter of distilled or deionized water.

2. If required, add 0.1–0.2% agar to prepare a semi-solid medium.

3. Heat to boiling with gentle agitation until completely dissolved.

4. Dispense into suitable containers.

5. Sterilize by autoclaving at 121 °C for 15 minutes.

6. Cool and store for use.