Sabouraud’s Broth Medium – 250g | Fungal Culture & Testing
Classic Acidic Medium for Fungal Cultivation and Testing
Sabouraud’s Broth Medium provides optimal conditions with high glucose concentration (40g/L) and acidic pH (5.6) to support robust fungal growth while inhibiting bacterial contamination. Ideal for both routine cultivation and antimicrobial efficacy testing.
Key Applications:
– Fungicidal efficacy testing
– Antifungal product evaluation
– Clinical mycology studies
– Environmental fungal isolation
– Pharmaceutical preservative testing
Technical Specifications:
– Formula (per liter):
– Peptone: 10.0g
– Dextrose: 40.0g
– pH: 5.6 ± 0.2 (25°C)
– Packaging: 250g/bottle
– Storage: Room temperature (15-25°C)
– Shelf Life: 3 years
Preparation Protocol:
1. Suspend 50.0g in 1L distilled water
2. Dissolve with gentle heating and stirring
3. Sterilize at 115°C for 30 minutes
Optional Enhancement:
For increased bacterial inhibition, cool to below 50°C and add chloramphenicol (10mg per 100ml) aseptically.
Quality Features:
– High dextrose content supports rapid fungal growth
– Acidic pH creates selective environment
– Optional antibiotic supplementation available
– Proven formula for reliable results