Sabouraud’s Broth Medium – 250g | Fungal Culture & Testing

Classic Acidic Medium for Fungal Cultivation and Testing

Sabouraud’s Broth Medium provides optimal conditions with high glucose concentration (40g/L) and acidic pH (5.6) to support robust fungal growth while inhibiting bacterial contamination. Ideal for both routine cultivation and antimicrobial efficacy testing.

Key Applications:

– Fungicidal efficacy testing

– Antifungal product evaluation

– Clinical mycology studies

– Environmental fungal isolation

– Pharmaceutical preservative testing

Technical Specifications:

– Formula (per liter):

– Peptone: 10.0g

– Dextrose: 40.0g

– pH: 5.6 ± 0.2 (25°C)

– Packaging: 250g/bottle

– Storage: Room temperature (15-25°C)

– Shelf Life: 3 years

Preparation Protocol:

1. Suspend 50.0g in 1L distilled water

2. Dissolve with gentle heating and stirring

3. Sterilize at 115°C for 30 minutes

Optional Enhancement:

For increased bacterial inhibition, cool to below 50°C and add chloramphenicol (10mg per 100ml) aseptically.

Quality Features:

– High dextrose content supports rapid fungal growth

– Acidic pH creates selective environment

– Optional antibiotic supplementation available

– Proven formula for reliable results