Key Features & Benefits:
- Enhanced Differentiation: The inclusion of TTC is reduced to red formazan by metabolically active microorganisms, staining fungal colonies red or pink. This significantly aids in visualizing and distinguishing colonies from media debris, improving counting accuracy.
- Classic Fungal Formulation: Based on the standard Sabouraud Dextrose Agar composition with a low pH of 5.6, optimal for dermatophytes, yeasts, and other fungi.
- Quantitative Analysis: Specifically suitable for the enumeration of fungi in quality control and environmental monitoring.
- Flexible Supplementation: Formulation allows for the optional addition of antibiotics (e.g., chloramphenicol) for enhanced bacterial inhibition.
Product Specifications
| Specification | Details |
|---|---|
| Product Type | Dehydrated Culture Media |
| Quantity | 250g (Prepares approximately 3.85 Liters of medium) |
| Application | Fungal cultivation and enumeration |
| Storage | Room temperature (Stable for 3 years) |
| Selective Agent | TTC (0.01g/L), Low pH 5.6 |
Composition (per liter)
- Peptone: 10.0g
- Dextrose (Glucose): 40.0g
- TTC (Triphenyl Tetrazolium Chloride): 0.01g
- Agar: 15.0g
Final pH: 5.6 ± 0.2 at 25°C
Instructions for Preparation
- Suspend 65.0g of the powder in 1 liter of distilled or deionized water.
- Heat and boil to dissolve the medium completely.
- Dispense into final containers.
- Sterilize by autoclaving at 121°C for 15 minutes or 115°C for 30 minutes.
- After sterilization, gently swirl the vessel to prevent agar sedimentation.
- Optional: For enhanced bacterial inhibition, cool the medium to approx. 50°C and aseptically add 1 vial of chloramphenicol (10mg) per 100ml of medium before pouring plates.