RPF Agar Medium Base for Coagulase-Positive Staphylococcus Detection Culture Medium Powder 250g
RPF Agar Medium Base
Culture Medium for Detection of Coagulase-Positive Staphylococcus
RPF Agar Medium Base is a microbiological culture medium designed for the detection and differentiation of coagulase-positive Staphylococcus species. The medium provides nutrients that support bacterial growth and allows the identification of characteristic reactions when supplemented with rabbit plasma, bovine fibrinogen, potassium tellurite, and trypsin inhibitor.
This medium is commonly used in food microbiology laboratories, public health laboratories, quality control laboratories, and microbiological research for the detection of coagulase-positive staphylococci.
The product is supplied in powder form, allowing convenient preparation and consistent laboratory performance.
Product Specifications
- Product Name: RPF Agar Medium Base
- Format: Powder Medium
- Net Weight: 250 g / bottle
- Storage: Store in a cool and dry place at room temperature
- Shelf Life: 3 years
- Application: Detection of coagulase-positive Staphylococcus
Composition (g/L)
Tryptone ……………………………………… 10.0
Beef Extract …………………………………. 5.0
Yeast Extract ……………………………….. 1.0
Sodium Pyruvate ………………………….. 10.0
Glycine ………………………………………….. 12.0
Lithium Chloride ……………………………. 5.0
Agar ……………………………………………… 15.0
pH 7.2 ± 0.2 (25°C)
Preparation Instructions
- Weigh 58 g of the medium powder.
- Dissolve in 900 mL of distilled water with heating until completely dissolved.
- Dispense and sterilize at 121°C for 15 minutes.
- After sterilization, mix well to prevent agar sedimentation and maintain in a 48°C water bath.
- Under aseptic conditions, add the following to each 90 mL of medium:
- 2.5 mg sterile potassium tellurite
- 2.5 mL rabbit plasma
- 7.5 mL bovine fibrinogen solution
- 2.5 mL trypsin inhibitor
- Mix gently and pour into sterile Petri dishes.
Preparation of Supplement Solutions
Bovine Fibrinogen Solution
Dissolve 5 g bovine fibrinogen in 100 mL distilled water and sterilize using 0.22 μm membrane filtration.
Trypsin Inhibitor Solution
Dissolve 30 mg trypsin inhibitor in 30 mL distilled water and sterilize using 0.22 μm membrane filtration.
Applications
- Detection of coagulase-positive Staphylococcus
- Food microbiology testing
- Quality control laboratories
- Microbial contamination monitoring
- Research laboratories