PEA Agar Base for Selective Gram-Positive Cocci Isolation
Selective Medium for Staphylococci & Streptococci Isolation
This PEA Agar Base requires supplementation with blood and β-phenylethanol to create a selective environment that suppresses Gram-negative bacteria while promoting Gram-positive cocci growth.
⚠️ Critical Preparation:
△ Must add 5-7% defibrinated sheep blood
△ Requires 0.25g/100ml β-phenylethanol
△ For clinical microbiology use only
Key Features:
Optimized casein/soy peptones for cocci recovery
Phenylethanol selectivity without antibiotic interference
Room temperature stable (3-year shelf life)
Complies with CLSI M35-A2 standards
Technical Specifications:
▶ Base Formula (per liter):
Pancreatic Digest of Casein 15.0g
Enzymatic Digest of Soybean Meal 5.0g
NaCl 5.0g | Agar 15.0g
▶ pH: 7.3±0.2 (25°C)
▶ Preparation:
40g/L → boil → sterilize at 121°C/15min
Cool 50-55°C → add 50ml sheep blood + 2.5g β-phenylethanol per liter
Diagnostic Applications:
Isolation of Staphylococcus aureus from mixed flora
Recovery of Enterococcus species
Wound/pus specimen processing
