Oxacillin Resistance Screening Agar Base for MRSA Detection | Dehydrated Culture Medium 250g

Oxacillin Resistance Screening Agar Base

Oxacillin Resistance Screening Agar Base is a microbiological culture medium formulated for the selective screening of methicillin-resistant Staphylococcus aureus (MRSA).

The medium contains mannitol and selective salts that support the differentiation and screening of resistant strains when supplemented with appropriate antimicrobial agents. After preparation and supplementation, the medium can be poured into agar plates for laboratory testing.

This dehydrated formulation offers consistent composition, convenient storage, and reliable performance for microbiology laboratories conducting MRSA surveillance and microbial analysis.

Key Features

• Designed for MRSA screening and detection
• Selective oxacillin resistance screening medium
• Dehydrated powder for easy storage and preparation
• Suitable for clinical microbiology and laboratory research
• Reliable and consistent microbiological culture medium
• Supports microbial screening and differentiation

Medium Composition (per liter)

Peptone …………………………………. 11.8 g
Yeast Extract ……………………………. 9.0 g
Mannitol …………………………………. 10.0 g
Sodium Chloride …………………………. 55.0 g
Lithium Chloride ………………………….. 5.0 g
Aniline Blue ………………………………. 0.2 g
Agar ……………………………………… 12.5 g

pH: 7.2 ± 0.2 (25°C)

Preparation Instructions

1. Suspend 103.5 g of the medium in 1 liter of distilled or deionized water.
2. Heat and boil until the medium is completely dissolved.
3. Dispense into suitable containers.
4. Sterilize in an autoclave at 121°C for 15 minutes.
5. After sterilization, mix well to prevent agar from settling at the bottom.
6. Cool the medium to approximately 55°C.
7. Under aseptic conditions, add polymyxin B (10,000 units) and oxacillin (0.4 mg) per 200 ml medium, mix thoroughly, and pour into agar plates.

Storage

Store tightly sealed in a cool and dry place at room temperature.
Shelf life: 3 years.

Product Specifications