MacConkey Broth – Selective Enrichment Medium for E.coli Detection
Premium MacConkey Broth – Gold Standard for E.coli Enrichment & Detection
Optimize your pathogen detection workflows with our selective MacConkey Broth – the definitive liquid medium for enhanced recovery and identification of coliform bacteria in food, water, and clinical samples. Formulated with bile salts and pH indicator, this enrichment broth delivers superior selectivity for Gram-negative bacteria per ISO 21528 and FDA BAM standards.
Critical Applications:
✔️ E.coli Pre-enrichment – Selective amplification before plating
✔️ Water Quality Testing – MPN method for coliform counts
✔️ Food Safety Analysis – Dairy/meat contamination screening
✔️ Clinical Specimen Processing – UTI pathogen enrichment
✔️ Pharmaceutical QC – Purified water system monitoring
Dual-Selective Formula (per liter):
| Component | Quantity | Function |
|—————————-|———-|——————————|
| Pancreatic Gelatin Digest | 20.0g | Nitrogen/Peptide Source |
| Lactose Monohydrate | 10.0g | Fermentation Substrate* |
| Dehydrated Ox Bile | 5.0g | Gram-positive Inhibition |
| Bromcresol Purple | 0.01g | pH Indicator (Color Change) |
pH: 7.3 ± 0.2 (25°C) – Optimized for enterobacterial growth
Positive: Purple→Yellow (Acid production) | Negative: Purple remains
Validated Preparation Protocol:
Dissolve 35.0g in 1L purified water
Gentle Heating – 50°C with stirring until clear
Distribute into tubes/bottles
Autoclave: 121°C for 15 minutes
Cool to room temperature before inoculation
