Plate Count Agar (PCA) - Standard Methods Medium for Total Aerobic Count

Plate Count Agar (PCA) – Standard Methods Medium for Total Aerobic Count

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Laboratory-Proven Plate Count Agar – Gold Standard for Aerobic Microbial Enumeration

Achieve accurate and reproducible bacterial counts with our premium Plate Count Agar (PCA) – the universally accepted medium for total viable count (TVC) testing in food, dairy, pharmaceutical, and water quality control. Formulated to ISO 4833 and FDA BAM standards, this nutrient-balanced agar delivers consistent colony development for reliable quality assessment.

Critical Applications:

  • ✔️ Food & Dairy Testing – ISO-compliant TVC enumeration

  • ✔️ Pharmaceutical QC – Raw material bioburden testing

  • ✔️ Water Quality Analysis – Drinking water heterotrophic count

  • ✔️ Cosmetic Microbiology – Preservation efficacy testing

  • ✔️ Environmental Monitoring – Surface contact plates

Optimized Formula (per liter):

| Component | Quantity | Function |

|—————————-|———-|——————————|

| High-Purity Tryptone | 5.0g | Primary Nitrogen Source |

| Yeast Extract | 2.5g | Vitamin Complex |

| Dextrose | 1.0g | Energy Substrate |

| Purified Agar | 15.0g | Optimal Gel Strength |

pH: 7.0 ± 0.2 (25°C) – Ideal for diverse aerobic microorganisms

Validated Preparation Protocol:

  1. Dissolve 23.5g in 1L purified water

  2. Boil with stirring until complete dissolution

  3. Autoclave: 121°C for 15 minutes

  4. Critical Step: Swirl gently post-sterilization

  5. Pour Plates at 45-50°C (4mm thickness)

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