Sealed bottle of Freshwater Fish Agar powder for aquaculture diagnostics

Freshwater Fish Agar (FWA) – Pathogen Detection Medium for Aquaculture (250g)

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Specialized Freshwater Fish Agar Medium for Aquatic Pathogen Isolation
Formulated specifically for detecting bacterial pathogens in freshwater fish, this agar combines balanced nutrients (5.0g/L peptone + 2.5g/L beef/yeast extracts) with essential minerals to support optimal growth of aquatic microorganisms. The pH-stabilized (7.3±0.1) formula ensures reliable results in fish disease diagnostics and hatchery health monitoring.

Critical Applications:

  • 🐟 Disease Diagnosis: Isolate Aeromonas, Pseudomonas and other fish pathogens

  • 🏥 Hatchery QC: Monitor bacterial loads in breeding facilities

  • 🔬 Research Studies: Investigate fish microbiome and antibiotic resistance

Technical Advantages:

  • Aquatic-Adapted Formula: Magnesium sulfate (0.05g/L) enhances pathogen recovery

  • Energy-Rich: 1.0g/L glucose supports fastidious bacteria

  • Osmotic Balance: 5.0g/L NaCl mimics fish physiological conditions

  • Ready-to-Prepare: 31.3g/L standardized concentration

Preparation Protocol:

  1. Suspend 31.3g in 1L purified water

  2. Boil until complete dissolution

  3. Autoclave at 121°C for 15 minutes

  4. Critical: Shake during cooling to prevent agar sedimentation

  5. Pour plates at ≈50°C

Complete Formulation (Per Liter):

  • Peptone: 5.0g

  • Beef Extract: 2.5g

  • Yeast Extract: 2.5g

  • Glucose: 1.0g

  • NaCl: 5.0g

  • MgSO₄: 0.05g

  • K₂HPO₄: 0.2g

  • Agar: 15.0g

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