Fluid Lactose Medium (Lactose Monohydrate Broth)

Fluid Lactose Medium, also known as Lactose Monohydrate Broth, is a dehydrated microbiological culture medium formulated for the detection of coliform bacteria and for pre-enrichment of Salmonella species in food, water, and environmental samples.

This medium contains gelatin pancreatic digest, beef extract, and lactose, providing essential nutrients and a fermentable carbohydrate source to support bacterial recovery and growth during preliminary testing stages.

⚠ For laboratory research and quality control use only. Not intended for clinical or therapeutic applications.

🔬 Intended Use

• Presumptive coliform testing

• Salmonella pre-enrichment procedures

• Food and water microbiological analysis

• Environmental and quality control laboratories

📦 Product Specifications

• Product Name: Fluid Lactose Medium

• Alternative Name: Lactose Monohydrate Broth

• Physical Form: Dehydrated powder

• Package Size: 250 g / bottle

• Storage Condition: Room temperature (RT)

• Shelf Life: 3 years

🧬 Medium Composition (per liter)

• Pancreatic Digest of Gelatin ………. 5.0 g

• Beef Extract …………………………………. 3.0 g

• Lactose ………………………………………….. 5.0 g

Final pH: 6.9 ± 0.2 (at 25 °C)

⚗️ Preparation Instructions

1. Suspend 13.0 g of dehydrated medium in 1 L of distilled or deionized water
   (volume may be adjusted proportionally as required)

2. Warm gently with stirring until completely dissolved

3. Sterilize at 121 °C for 15 minutes

4. Cool rapidly after sterilization

5. Store under appropriate conditions until use

🧫 Storage & Stability

• Store at room temperature

• Keep container tightly sealed

• Protect from moisture and excessive heat