Fluid Casein Digest–Soy Lecithin–Polysorbate 20 Medium Base

Fluid Casein Digest–Soy Lecithin–Polysorbate 20 Medium Base is a dehydrated culture medium base designed for sample dilution and neutralization in pharmaceutical microbiological testing. When supplemented with Polysorbate 20 (Tween 20), it is commonly used for drug product dilution, preservative neutralization, and microbial recovery procedures.

The formulation contains casein enzymatic digest and soy lecithin, providing effective neutralization capacity while maintaining microbial viability during sample preparation.

⚠ For laboratory research and industrial quality control use only. Not for clinical or therapeutic use.

🔬 Intended Use

• Pharmaceutical sample dilution

• Preservative neutralization testing

• Microbial recovery during microbiological analysis

• Quality control and routine laboratory testing

📦 Product Specifications

• Product Name: Fluid Casein Digest–Soy Lecithin–Polysorbate 20 Medium Base

• Physical Form: Dehydrated powder

• Package Size: 250 g / bottle

• Storage Condition: Room temperature (RT)

• Shelf Life: 3 years

🧬 Medium Composition (per liter)

• Pancreatic Digest of Casein ………… 20.0 g

• Lecithin ………………………………………… 5.0 g

Final pH: 7.3 ± 0.2 (at 25 °C)

Note: Polysorbate 20 (Tween 20) is not included in the base medium and must be added separately during preparation.

⚗️ Preparation Instructions

1. Suspend 25.0 g of dehydrated medium in 960 mL of purified or deionized water

2. Add 40 mL of Polysorbate 20 (Tween 20)

3. Dissolve in a 48–50 °C water bath for 30 minutes, with gentle mixing

4. Dispense into suitable containers

5. Sterilize at 121 °C for 15 minutes

6. Cool and store for use