Dey/Engley Neutralizing Agar Base (D/E Agar Base)

Dey/Engley Neutralizing Agar Base is a dehydrated solid culture medium designed for cosmetic sample preparation and microbial cultivation after neutralization of preservatives and disinfectants.

This agar-based formulation is commonly used in cosmetic microbiology to neutralize residual antimicrobial substances during sample handling, allowing accurate recovery, cultivation, and enumeration of microorganisms.
The medium provides a stable solid surface suitable for routine microbiological testing in cosmetic, personal care, and laboratory quality control applications.

🔬 Product Applications

• Preparation of cosmetic and personal care samples

• Neutralization of preservatives and disinfectants

• Cultivation and enumeration of microorganisms

• Cosmetic microbiology testing

• Routine quality control and laboratory analysis

📦 Product Specifications

• Product Name: Dey/Engley Neutralizing Agar Base

• English Name: Dey/Engley Neutralizing Agar Base

• Form: Dehydrated powder

• Package Size: 250 g / bottle

• Storage Condition: Room temperature, dry and tightly sealed

• Shelf Life: 3 years

🧾 Medium Composition (per liter)

• Pancreatic Digest of Casein – 5.0 g

• Glucose – 10.0 g

• Soybean Lecithin – 7.0 g

• Sodium Thiosulfate – 6.0 g

• Sodium Hydrogensulfite – 2.5 g

• Yeast Extract – 2.5 g

• Sodium Thioglycollate – 1.0 g

• Bromcresol Purple – 0.02 g

• Agar – 15.0 g

Final pH: 7.6 ± 0.2 (25 °C)

⚙️ Directions for Use

1. Suspend 49.0 g of the medium in 1 liter of distilled or deionized water.

2. Heat with stirring and boil for at least 1 minute until completely dissolved.

3. Dispense into suitable containers.

4. Add 1 g Tween 80 per 200 ml of medium.

5. Sterilize by autoclaving at 121 °C for 15 minutes.

6. Cool and use as required.