Chocolate Blood Agar Medium Base – 250g for Cultivation of Neisseria and Haemophilus

Chocolate Blood Agar Medium Base is a nutrient-rich culture medium designed for the cultivation of fastidious organisms, particularly Neisseria species and Haemophilus influenzae. When supplemented with defibrinated sheep blood, the medium undergoes controlled heating to release growth factors (X and V factors) from red blood cells, producing the characteristic chocolate-brown appearance essential for optimal bacterial recovery.

Product Information

• Product Name: Chocolate Blood Agar Medium Base

• Pack Size: 250g per bottle

• Form: Dehydrated culture medium powder

• Storage: Store at room temperature

• Shelf Life: 3 years

Applications

• Cultivation of Neisseria gonorrhoeae and Neisseria meningitidis

• Isolation of Haemophilus influenzae

• Clinical microbiology and diagnostic laboratories

• Research involving fastidious bacterial pathogens

Key Features

• Provides essential nutrients for fastidious organisms

• Supports release of hemin (X factor) and NAD (V factor) after heating with blood

• Produces characteristic chocolate-brown medium for accurate identification

• Widely used in clinical and research applications

Medium Composition (per liter)

• Peptone: 15.0 g

• Beef Extract: 3.0 g

• Yeast Extract: 4.0 g

• Maize Starch: 1.0 g

• Sodium Chloride: 5.0 g

• Agar: 15.0 g

• Final pH: 7.2 ± 0.2 (25℃)

Preparation Instructions

1. Suspend 43.0 g of the medium in 1 L distilled or deionized water.

2. Heat to boiling until fully dissolved.

3. Dispense into containers and autoclave at 121℃ for 15 minutes.

4. After sterilization, maintain at ~90℃ and aseptically add 5–10% defibrinated sheep blood.

5. Mix gently and continue heating with shaking until hemolysis occurs and the medium turns chocolate-brown.

6. Cool to ~50℃, mix thoroughly, and pour plates.