Buffered Charcoal Yeast Extract Agar Base without L-cysteine | BCYE-Cys Medium

Overview

Buffered Charcoal Yeast Extract Agar Base without L-cysteine (BCYE-Cys Agar Base) is a dehydrated culture medium designed for the isolation and identification of Legionella species.
It provides a selective medium without L-cysteine, making it suitable for differential testing and confirmatory diagnosis of Legionella in microbiological laboratories.

Specifications

• Form: Dehydrated culture medium

• Appearance: Fine powder

• Packing Size: 100 g/bottle

• Storage Condition: Room temperature

• Shelf Life: 3 years

Formula (per liter)

• Activated Charcoal ……………………………. 2.0 g

• Yeast Extract …………………………………….. 10.0 g

• ACES Buffer ……………………………………… 10.0 g

• α–Ketoglutarate …………………………………. 1.0 g

• Agar …………………………………………………. 15.0 g

• Final pH: 6.85 ± 0.2 (at 25 °C)

Preparation and Usage

1. Suspend 38.0 g of the medium in 1 L distilled or deionized water.

2. Heat with frequent agitation until completely dissolved.

3. Dispense in 100 ml volumes and autoclave at 121 °C for 15 minutes.

4. Shake gently after sterilization and allow to cool to 50 °C.

5. Aseptically add 1 vial of soluble ferric pyrophosphate per 100 ml of medium and mix well.

6. Pour into sterile Petri dishes under aseptic conditions.

7. Adjust pH with sterile KOH solution if necessary.

Key Features

• Formulated for Legionella isolation and confirmation.

• L-cysteine-free composition for differential testing.

• Can be used alongside BCYE agar with L-cysteine for comparison.