Bromocresol Purple Broth Base for Custom Fermentation Tests
Versatile Purple Broth for Bacterial Metabolism Studies
This base medium with bromocresol purple indicator allows customizable carbohydrate fermentation testing when supplemented with 0.5-1% carbon sources. Ideal for acid/gas production analysis with Durham tubes.
Key Features:
Pre-dissolved pH indicator for instant color transition (purple→yellow)
Compatible with all fermentable sugars (glucose/sucrose/lactose/etc.)
Room temperature stable (3-year shelf life)
Optimized electrolyte balance for microbial growth
Technical Parameters:
▶ Formula (per liter):
Gelatin Peptone 10.0g | NaCl 5.0g
Bromocresol Purple 0.02g
▶ pH: 6.6-7.0 (25°C)
▶ Preparation:
15g/L distilled water + 5-10g/L carbohydrate
Adjust pH → dispense with Durham tubes
Sterilize at 115°C for 20min
Critical Applications:
Differentiation of Enterobacter and Klebsiella
Water/food safety testing
Educational microbiology experiments
