Brilliant Green-Agar Medium (250g) for selective isolation of Salmonella in pharmaceutical products

Brilliant Green Agar Medium

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Brilliant Green Agar Medium is a selective solid culture medium designed for the isolation of Salmonella species, particularly in pharmaceutical microbiological testing.

The presence of brilliant green dye, combined with phenol red indicator and fermentable carbohydrates (lactose and sucrose), inhibits most Gram-positive bacteria and many Gram-negative organisms while allowing Salmonella to grow with characteristic colony appearance.

⚠ For laboratory research and quality control use only. Not intended for clinical diagnostic or therapeutic use.

🔬 Intended Use

  • Selective isolation of Salmonella

  • Microbiological testing of pharmaceutical products

  • Quality control laboratories

  • Regulatory and compliance testing

📦 Product Specifications

  • Product Name: Brilliant Green Agar Medium

  • Physical Form: Dehydrated powder

  • Package Size: 250 g / bottle

  • Storage Condition: Room temperature, dry place

  • Shelf Life: 3 years

🧬 Medium Composition (per liter)

  • Pancreatic Digest of Casein ……………………. 5.0 g
  • Yeast Extract ………………………………………… 3.0 g
  • Peptic Digest of Animal Tissue ……………….. 5.0 g
  • Lactose …………………………………………………….. 10.0 g
  • Sucrose …………………………………………………….. 10.0 g
  • Sodium Chloride ………………………………………. 5.0 g
  • Phenol Red ……………………………………………… 0.08 g
  • Brilliant Green …………………………………………. 0.0125 g
  • Agar ……………………………………………………….. 20.0 g
  • Final pH: 6.9 ± 0.2 (at 25 °C)

⚗️ Preparation Instructions

  1. Suspend 58.0 g of dehydrated medium in 1,000 ml of distilled or deionized water
  2. Heat to boiling with frequent stirring until completely dissolved
  3. Dispense into suitable containers
  4. Sterilize by autoclaving at:
    • 121 °C for 15 minutes, or
    • 115 °C for 30 minutes
  5. Cool to approximately 55 °C
  6. Pour into sterile Petri dishes

🧫 Storage & Stability

  • Store dehydrated medium at room temperature, protected from moisture and light
  • Do not use medium showing caking, discoloration, or moisture absorption

⚠️ Limitations & Precautions

  • Overheating may reduce selectivity
  • Avoid prolonged exposure to light due to dye sensitivity
  • For non-clinical laboratory use only

Brilliant Green Agar Medium (USP Standard) & Related Products

Product NameSpecificationDescription & Application
Hayflick Broth Medium (USP Standard)250gUsed for the enrichment culture of Mycoplasma.
Hayflick Agar Medium (USP Standard)250gUsed for the isolation culture of Mycoplasma
Fluid K Solution (USP 35 Standard)250gUsed as a rinse solution for sample preparation
RV Salmonella Enrichment Broth Tube (USP)10ml*20 tubes/boxUsed for the selective enrichment culture of Salmonella

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