Brain Heart Infusion Broth (BHI Broth)

Brain Heart Infusion Broth (BHI Broth) is a nutritionally rich, dehydrated culture medium formulated for the cultivation of a wide range of fastidious and non-fastidious microorganisms.
The medium contains balanced amounts of calf brain infusion and beef heart infusion, providing essential nitrogenous compounds, vitamins, and growth-supporting nutrients.

BHI Broth is widely used in microbiology laboratories for routine bacterial culture, enrichment, and propagation. Its high nutrient content supports excellent microbial growth performance and consistency across laboratory applications.

The dehydrated powder format ensures easy preparation, stable quality, and long shelf life.

🔬 Product Applications

• General bacterial cultivation

• Enrichment of fastidious organisms

• Clinical microbiology laboratories

• Food, pharmaceutical, and research testing

• Routine laboratory culture and propagation

📦 Product Specifications

• Product Name: Brain Heart Infusion Broth

• English Name: Brain Heart Infusion Broth

• Also Known As: BHI Broth

• Form: Dehydrated powder

• Package Size: 250 g / bottle

• Storage Condition: Room temperature, dry place

• Shelf Life: 3 years

🧾 Medium Composition (per liter)

• Dehydrated Calf Brain Infusion – 4.0 g

• Dehydrated Beef Heart Infusion – 4.0 g

• Pancreatic Digest of Casein – 16.0 g

• Peptone – 5.0 g

• Dextrose – 2.0 g

• Sodium Chloride – 5.0 g

• Disodium Phosphate (Na₂HPO₄) – 2.5 g

Final pH: 7.4 ± 0.2 (25 °C)

⚙️ Directions for Use

1. Suspend 38.5 g of the medium in 1 liter of distilled or deionized water.

2. Heat with stirring until completely dissolved.

3. Dispense into suitable containers.

4. Sterilize by autoclaving at 121 °C for 15 minutes.

5. Cool and store appropriately until use.