Bismuth Sulfite Agar

Bismuth Sulfite Agar (BS Agar) is a highly selective solid microbiological culture medium designed for the isolation and differentiation of Salmonella species, particularly Salmonella Typhi, from food, water, and environmental samples.

🔬 Intended Use

• Selective isolation of Salmonella spp.

• Food microbiology testing

• Water quality analysis

• Environmental and quality control laboratories

📦 Product Specifications

• Product Name: Bismuth Sulfite Agar

• Physical Form: Dehydrated powder

• Package Size: 250 g / bottle

• Storage Condition: Room temperature (RT)

• Shelf Life: 3 years

🧬 Medium Composition (per liter)

• Peptone ………………………………………….. 10.0 g

• Beef Extract ……………………………………… 5.0 g

• Dextrose ………………………………………….. 5.0 g

• Ferrous Sulfate ………………………………… 0.3 g

• Disodium Hydrogen Phosphate …….. 4.0 g

• Ammonium Bismuth Citrate ………. 2.0 g

• Brilliant Green ……………………………….. 0.025 g

• Sodium Sulfite ………………………………….. 6.0 g

• Agar …………………………………………………. 18.0 g

Final pH: 7.5 ± 0.2 (at 25 °C)

⚗️ Preparation Instructions

1. Suspend 5.03 g of dehydrated medium in 100 ml of distilled or deionized water

2. Heat with stirring and boil for 1 minute until fully dissolved

3. Adjust volume if required

4. Cool to 50–55 °C

5. Pour into sterile Petri dishes