🧪 Medium Composition (per liter)
- Casein Amino Acids (for vitamin assay) …… 12.0 g
- Glucose …… 40.0 g
- Sodium Acetate …… 20.0 g
- L-Cystine …… 0.2 g
- DL-Tryptophan …… 0.2 g
- Adenine Sulfate …… 20 mg
- Guanine Hydrochloride …… 20 mg
- Uracil …… 20 mg
- Thiamine Hydrochloride …… 2 mg
- Riboflavin …… 2 mg
- Nicotinic Acid …… 2 mg
- Calcium Pantothenate …… 2 mg
- Pyridoxine Hydrochloride …… 4 mg
- p-Aminobenzoic Acid …… 200 μg
- Magnesium Sulfate …… 0.4 g
- Dipotassium Phosphate …… 1.0 g
- Monopotassium Phosphate …… 1.0 g
- Sodium Chloride …… 20 mg
- Ferrous Sulfate …… 20 mg
- Manganese Sulfate …… 20 mg
Final pH: 6.8 ± 0.2 (25°C)
⚗️ Preparation Instructions
- Dissolve 5.49 g of medium base and 2.0 g of supplement (sodium acetate) in 100 ml of distilled or deionized water.
- Heat gently to dissolve completely.
- Cool to room temperature.
- Dispense aseptically into suitable containers for further use.
Preparation and assay procedures should follow validated microbiological assay protocols or relevant pharmacopeial methods.
🧫 Storage & Stability
- Store dehydrated medium at room temperature, protected from moisture and light
- Do not use if the medium shows caking, discoloration, or contamination
⚠️ Limitations & Precautions
- For vitamin assay use only
- Assay accuracy depends on correct strain selection and standard curve preparation
- Follow applicable pharmacopeial or regulatory guidelines