Clear liquid bacterial culture broth in test tubes with fermentation color gradient

Andrade’s Broth Base for Custom Carbohydrate Fermentation

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Classic Formulation for Bacterial Sugar Metabolism Studies
This time-tested Andrade’s Broth Base enables precise fermentation profiling when supplemented with specific carbohydrates (0.5-1%). Features acid-sensitive indicator for reliable pH shift detection.

Key Advantages:

  • Proven formula for enteric bacteria differentiation

  • Flexible carbon source compatibility

  • Room temperature stable (3-year shelf life)

  • Streamlined single-component preparation

Technical Application:
▶ Standard Protocol:

  1. Dissolve per manufacturer’s instructions

  2. Add 5-10g/L target carbohydrate

  3. Sterilize at 115-121°C for 15-20min
    ▶ Visual Interpretation:
    Color transition indicates acid production

Critical Use Cases:

  • IMViC test component for E. coli identification

  • Pathogen metabolism research

  • Water quality testing

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