3% NaCl Tryptone Soy Agar (TSA) – 250g | Vibrio parahaemolyticus Purification Medium

3% NaCl Tryptone Soy Agar is a high-salinity growth medium optimized for purification and maintenance of Vibrio parahaemolyticus after selective isolation on TCBS agar, providing essential nutrients for halophilic bacteria without competitive inhibitors.

Key Applications:

✔️ Purification of V. parahaemolyticus from mixed cultures

✔️ Subculturing suspicious colonies from TCBS agar

✔️ Biomass production for biochemical testing

✔️ Long-term strain maintenance

Halophilic Formulation (Per Liter):

| Component | Quantity |

|——————————-|———-|

| Tryptone | 15.0 g |

| Enzymatic Digest of Soybean Meal | 5.0 g |

| Sodium Chloride | 30.0 g |

| Agar | 15.0 g |

Final pH: 7.3 ± 0.2 (25°C)

Critical Protocol Notes:

1️⃣ Suspend 65.0g powder in 1L distilled water

2️⃣ Boil ≥1 minute with continuous stirring

3️⃣ Autoclave at 121°C for 15 minutes

4️⃣ Pour plates at 45-50°C

Technical Advantages:

– Enhanced halotolerance: 3% NaCl mimics marine environment for optimal vibrio growth

– Dual nitrogen sources: Tryptone + soybean digest support rapid biomass production

– Neutral pH stability: Maintains conditions for enzymatic activity

– Ready-to-use powder: Ensures consistent gel strength

– 3-year shelf life: Moisture-resistant packaging

Workflow Integration:

– Compatible with TCBS agar → Biochemical test workflow

– Validated for API 20E/20NE identification systems