3% NaCl Tryptone Soy Agar (TSA) – 250g | Vibrio parahaemolyticus Purification Medium
3% NaCl Tryptone Soy Agar is a high-salinity growth medium optimized for purification and maintenance of Vibrio parahaemolyticus after selective isolation on TCBS agar, providing essential nutrients for halophilic bacteria without competitive inhibitors.
Key Applications:
✔️ Purification of V. parahaemolyticus from mixed cultures
✔️ Subculturing suspicious colonies from TCBS agar
✔️ Biomass production for biochemical testing
✔️ Long-term strain maintenance
Halophilic Formulation (Per Liter):
| Component | Quantity |
|——————————-|———-|
| Tryptone | 15.0 g |
| Enzymatic Digest of Soybean Meal | 5.0 g |
| Sodium Chloride | 30.0 g |
| Agar | 15.0 g |
Final pH: 7.3 ± 0.2 (25°C)
Critical Protocol Notes:
1️⃣ Suspend 65.0g powder in 1L distilled water
2️⃣ Boil ≥1 minute with continuous stirring
3️⃣ Autoclave at 121°C for 15 minutes
4️⃣ Pour plates at 45-50°C
Technical Advantages:
– Enhanced halotolerance: 3% NaCl mimics marine environment for optimal vibrio growth
– Dual nitrogen sources: Tryptone + soybean digest support rapid biomass production
– Neutral pH stability: Maintains conditions for enzymatic activity
– Ready-to-use powder: Ensures consistent gel strength
– 3-year shelf life: Moisture-resistant packaging
Workflow Integration:
– Compatible with TCBS agar → Biochemical test workflow
– Validated for API 20E/20NE identification systems
