10% Sodium Chloride Tryptic Soy Broth – Enrichment Medium for Staphylococcus aureus and MPN Testing (250g)

10% Sodium Chloride Tryptic Soy Broth (10% NaCl TSB) is a selective enrichment medium designed for the growth and detection of Staphylococcus aureus, especially in Most Probable Number (MPN) tests. The high salt concentration (10% NaCl) effectively suppresses the growth of competing bacteria, while the enriched nutrients in tryptic soy broth support the selective proliferation of staphylococci.

Key Applications:

• Enrichment of Staphylococcus aureus in food, cosmetic, water, and clinical samples

• Most Probable Number (MPN) method for quantitative microbial testing

• Selective cultivation of staphylococci in microbiology laboratories

Composition (per liter):

• Pancreatic Digest of Casein (Tryptone) – 17.0 g

• Enzymatic Digest of Soybean Meal – 3.0 g

• Sodium Chloride – 100.0 g

• Sodium Pyruvate – 10.0 g

• Dipotassium Phosphate – 2.5 g

• Dextrose – 2.5 g

• Final pH: 7.3 ± 0.2 (at 25°C)

Preparation & Instructions:

1. Suspend 135.0 g of the medium in 1 liter of distilled or deionized water.

2. Heat until completely dissolved.

3. Dispense into appropriate containers.

4. Sterilize by autoclaving at 121°C for 15 minutes.

5. Cool and store until use.