Burkholderia cepacia Agar Plates – Ready-to-Use Selective Culture Medium
Product Overview
Burkholderia cepacia Agar Plates are ready-to-use solid culture media designed for the selective isolation of Burkholderia cepacia in microbiological testing.
This prepared agar medium contains peptone, yeast extract, sodium pyruvate, mineral salts, bile salts, and selective antimicrobial agents. Phenol red serves as a pH indicator, while crystal violet contributes to the selective properties of the medium.
The prepared plates provide a convenient format for laboratory microbiology applications, helping reduce the preparation steps associated with dehydrated culture media.
Each plate has a diameter of 90 mm and contains approximately 20–25 mL of prepared medium. The product is supplied in packs of 10 plates.
Key Product Features
- Ready-to-Use Format: Pre-poured agar plates for convenient laboratory use.
- Selective Culture Medium: Formulated for the selective isolation of Burkholderia cepacia.
- Standard Plate Size: 90 mm diameter Petri dishes.
- Consistent Filling Volume: Approximately 20–25 mL of medium per plate.
- Defined Formulation: Contains peptone, yeast extract, sodium pyruvate, mineral salts, and selective agents.
- Quality-Controlled Production: Subjected to sterility and performance testing.
- Convenient Packaging: Supplied as 10 plates per pack.
Intended Use
This product is intended for the selective isolation of Burkholderia cepacia in microbiological testing and laboratory research applications.
It is designed for use by trained laboratory personnel in accordance with established laboratory procedures and applicable biosafety requirements.
For microbiological testing only. Not intended for clinical diagnosis or direct medical use.
Product Specifications
| Parameter | Specification |
|---|---|
| Product Name | Burkholderia cepacia Agar Plates |
| Medium Type | Selective Solid Agar Medium |
| Physical Form | Prepared Agar Plates |
| Plate Diameter | 90 mm |
| Medium Volume | 20–25 mL per plate |
| Packaging | 10 plates per pack |
| Appearance | Orange-yellow |
| pH | 7.2 ± 0.2 at 25°C |
| Storage Temperature | 2–8°C |
| Storage Conditions | Protect from light |
| Shelf Life | 3 months |
| Intended Application | Selective isolation of Burkholderia cepacia |
| Sterility | Sterility tested |
| Product Use | Microbiological testing |
Medium Composition
The formulation contains the following ingredients:
| Ingredient | Amount |
|---|---|
| Peptone | 5.0 g |
| Yeast Extract | 4.0 g |
| Sodium Pyruvate | 7.0 g |
| Potassium Dihydrogen Phosphate | 4.4 g |
| Disodium Hydrogen Phosphate | 1.4 g |
| Bile Salts | 1.5 g |
| Ammonium Sulfate | 1.0 g |
| Magnesium Sulfate | 0.2 g |
| Ammonium Ferrous Sulfate | 0.01 g |
| Phenol Red | 0.02 g |
| Crystal Violet | 0.001 g |
| Agar | 12.0 g |
| Polymyxin B | 75,000 IU |
| Gentamicin | 2.5 mg |
| Ticarcillin | 50.0 mg |
| Distilled Water | 1,000 mL |
| Final pH | 7.2 ± 0.2 at 25°C |
Quality Control and Performance Specifications
Each batch is evaluated according to the following product quality requirements:
1. Appearance
The prepared medium should have an orange-yellow appearance, with no visible insoluble particles or cracks.
2. Physicochemical Properties
- pH: 7.0–7.4
- Gel strength: 500–650 g/cm²
3. Sterility
The medium is tested for sterility and should show no microbial growth during the specified sterility test.
4. Growth Performance
- Burkholderia cepacia: Expected to produce gray-green colonies, with a pink color change in the surrounding medium.
- Pseudomonas aeruginosa: Growth inhibition is expected under the specified product performance test conditions.
These performance characteristics are based on the manufacturer’s stated quality control specifications. Actual results may depend on the strain, inoculum, and testing conditions.
Directions for Use
- Remove the agar plates from refrigerated storage.
- Allow the plates to equilibrate to room temperature before use.
- Perform inoculation or testing according to the validated laboratory procedure.
- After inoculation, transfer the plates to the designated incubation environment and incubate under the validated conditions specified by the applicable test method.
- Observe and record the resulting microbial growth according to the laboratory’s testing protocol.
Use appropriate microbiological techniques and applicable biosafety procedures throughout the testing process.
Storage and Shelf Life
- Storage Temperature: 2–8°C.
- Keep away from direct light.
- Store the plates in their original packaging.
- Avoid significant temperature fluctuations exceeding 10°C, as these may cause condensation.
- Recommended shelf life: 3 months from the manufacturing date, subject to the product label and storage requirements.
For better moisture management, allow the plates to stand upright in the working environment for approximately 4–6 hours before use, as appropriate for the laboratory procedure.
Small amounts of condensation or occasional water droplets inside the packaging may occur during storage and do not necessarily indicate product failure. However, plates showing contamination, cracks, or significant drying should not be used.
Important Precautions
- The product is sterilized by autoclaving and aseptically filled during manufacturing.
- Do not use plates showing visible contamination, cracks, or excessive drying before use.
- Avoid temperature differences greater than 10°C during storage and handling to minimize condensation.
- This product is intended for single use only.
- After use, dispose of the plates following appropriate validated decontamination procedures, including autoclaving where applicable, and comply with local biological waste regulations.
- For microbiological testing by trained personnel only.
- This product is not intended for clinical diagnosis, therapeutic use, or direct human or veterinary use.
Packaging Information
- Package Format: 10 prepared agar plates per pack.
- Plate Diameter: 90 mm.
- Medium Volume: 20–25 mL per plate.
- Product Form: Ready-to-use solid culture medium.
Frequently Asked Questions
Q1: What is Burkholderia cepacia Agar used for?
It is a selective agar medium intended for the isolation of Burkholderia cepacia in microbiological testing.
Q2: Is this product supplied as dehydrated powder?
No. This product is supplied as pre-prepared, ready-to-use agar plates.
Q3: What is the plate size?
Each prepared agar plate has a diameter of 90 mm.
Q4: How many plates are included in one pack?
Each pack contains 10 prepared agar plates.
Q5: How should the product be stored?
Store at 2–8°C and protect from light. Avoid significant temperature fluctuations.
Q6: What is the shelf life?
The stated shelf life is 3 months, subject to the manufacturing date and storage requirements.
Q7: Is this product suitable for clinical diagnosis?
No. This product is intended for microbiological testing only and is not intended for clinical diagnosis.
Product Notice: Product specifications and performance characteristics are provided for laboratory reference. Please refer to the product label and accompanying technical documentation for batch-specific information. This product is intended for professional laboratory use only.
