Product Overview
This Cetrimide Agar Plate is a ready-to-use, selective medium designed for the isolation and cultivation of Pseudomonas aeruginosa from clinical, environmental, and industrial samples. The selective agent, cetrimide (cetyltrimethylammonium bromide), is a quaternary ammonium compound that inhibits the growth of most non-Pseudomonas organisms while permitting P. aeruginosa to grow.
The medium also contains peptone and beef extract to supply the nutrients required for growth of Pseudomonas species. Cetrimide Agar is widely used in pharmaceutical, cosmetic, and clinical microbiology for the detection and enumeration of P. aeruginosa, and it is referenced in several pharmacopoeial and national standards.
Intended Use
- Selective isolation and cultivation of Pseudomonas aeruginosa.
- Microbiological examination of pharmaceutical, cosmetic, and environmental samples.
- Hygiene monitoring and quality control testing.
- Educational and research laboratory use.
Composition (per Liter)
- Peptone: 1.0 g
- Beef Extract: 0.3 g
- Sodium Chloride: 0.5 g
- Cetrimide (Cetyltrimethylammonium Bromide): 0.03 g
- Agar: 2.0 g
- Final pH: 7.4–7.6 at 25°C
Performance Characteristics
- Appearance: Light yellow with a small amount of insoluble material.
- Physicochemical: pH 7.4–7.6.
- Sterility: No bacterial growth after incubation at 36 ± 1°C for 48 hours.
- Growth Promotion: Validated for robust growth of Pseudomonas aeruginosa after incubation at 36 ± 1°C for 18–24 hours.
Packaging and Storage
- Format: 90mm × 10 plates per pack.
- Fill Volume: 20–25 mL per plate.
- Packaging: Vacuum packaging for controlled environment transfer.
- Shelf Life: 30 days from date of manufacture.
- Storage: Store at 2–8°C, protected from light. Do not freeze.
