Product Overview
This Columbia Agar Plate is a ready-to-use, general-purpose microbiological medium designed for the isolation and cultivation of Clostridium species and other fastidious microorganisms. Columbia Agar Base is widely recognized as one of the most nutritious general-purpose media available, supporting the growth of a broad range of bacteria, including anaerobic and aerobic organisms.
The medium combines multiple peptone sources — pancreatic digest of casein, peptic digest of animal tissue, yeast extract, heart pancreatic digest, and corn starch — to provide a rich supply of nutrients, vitamins, and growth factors. The corn starch acts as a protective agent and supports the growth of fastidious organisms. This formulation is particularly suitable for anaerobic culture and is commonly used as a base for blood-supplemented media.
The 90mm format is suitable for streaking, swabbing, and general inoculation procedures, and the triple sterile packaging allows safe transfer into controlled environments.
Intended Use
- Isolation and cultivation of Clostridium species.
- General-purpose culture of fastidious and non-fastidious microorganisms.
- Anaerobic and aerobic microbiological examination.
- Environmental monitoring in pharmaceutical and biotechnology facilities.
- Educational and research laboratory use.
Composition (per Liter)
- Pancreatic Digest of Casein: 10.0 g
- Sodium Chloride: 5.0 g
- Peptic Digest of Animal Tissue: 5.0 g
- Yeast Extract: 5.0 g
- Heart Pancreatic Digest: 3.0 g
- Corn Starch: 1.0 g
- Agar: 15.0 g
- Purified Water: 1,000 mL
- Final pH: 7.3 ± 0.2 at 25°C
Performance Characteristics
- Appearance: Light yellow, smooth surface, free from cracks, visible impurities, and bubbles.
- Physicochemical: pH 7.1–7.5; gel strength 500–650 g/cm².
- Sterility: No growth after 2 days of incubation.
- Growth Promotion: Validated for robust growth with typical colony size and morphology for Clostridium sporogenes and Clostridium perfringens.
Packaging and Storage
- Format: 90mm × 10 plates per pack.
- Fill Volume: 20–25 mL per plate.
- Packaging: Three-layer sterile packaging for controlled environment transfer.
- Shelf Life: 3 months from date of manufacture.
- Storage: Store at 2–25°C, protected from light. Do not freeze.
