SCDLP Broth Base for Microbial Enrichment

SCDLP Broth Base (Soya Casein Digest Lecithin Polysorbate Broth Base) is a dehydrated enrichment medium designed for microbial recovery and sample enrichment, particularly in the presence of inhibitory substances.

This medium is commonly used as a base formulation and requires the addition of Polysorbate 80 (Tween 80) prior to sterilization. Its balanced nutrient system supports the growth of a broad range of microorganisms while maintaining stability during routine laboratory workflows.

The powdered format ensures convenient preparation, consistent quality, and long-term storage, making it suitable for microbiology laboratories involved in product testing, environmental monitoring, and routine microbial enrichment.

🔬 Applications

• Microbial enrichment from test samples

• Recovery of stressed or low-level microorganisms

• Routine microbiological sample preparation

• General laboratory enrichment culture

📦 Product Specifications

• Product Name: SCDLP Broth Base

• English Name: Soya Casein Digest Lecithin Polysorbate Broth Base

• Form: Dehydrated powder

• Package Size: 250 g / bottle

• Storage Condition: Room temperature, dry place

• Shelf Life: 3 years

🧾 Medium Composition (per liter)

• Pancreatic Digest of Casein – 17.0 g

• Enzymatic Digest of Soybean Meal – 3.0 g

• Dipotassium Phosphate – 2.5 g

• Sodium Chloride – 5.0 g

• Dextrose – 2.5 g

• Lecithin – 1.0 g

Final pH: 7.2 ± 0.2 (25 °C)

⚙️ Directions for Use

1. Suspend 31.0 g of the medium in 1 liter of distilled or deionized water.

2. Add 7.0 g of Polysorbate 80 (Tween 80) per liter
   (or 0.7 g per 100 mL of medium).

3. Heat gently with stirring until completely dissolved.

4. Dispense into suitable containers.

5. Sterilize by autoclaving at 121 °C for 20 minutes.

6. Cool to room temperature before use.