Magnetic Spirillum Growth Medium, Revised (MSGM, Revised)

Magnetic Spirillum Growth Medium, Revised (MSGM, Revised) is a specialized medium formulated for the cultivation of magnetotactic bacteria, including those containing magnetosomes. The medium provides essential minerals, vitamins, and organic acids to support selective bacterial growth.

Applications:

• Cultivation of magnetotactic bacteria

• Microbial research on magnetic microorganisms

• Laboratory enrichment of magnetosome-producing strains

Specifications:

• Format: Powder, 250 g/bottle

• Storage: Room temperature

• Shelf Life: 3 years

Formula (per liter):

• Agar – 1.3 g

• KH2PO4 – 0.68 g

• Tartaric acid – 0.37 g

• Succinic acid – 0.37 g

• Sodium salts – 0.12 g

• Sodium acetate – 0.05 g

• Ascorbic acid – 0.035 g

• Wolfe’s vitamin solution – 10.0 ml

• Wolfe’s mineral solution – 5.0 ml

• Ferric quinate solution – 2.0 ml

• Resazurin (0.1% solution) – 0.45 ml

• Final pH: 6.75 ± 0.2 (25℃)

Preparation Instructions:

1. Dissolve 3.0 g of the medium in 1 liter of distilled or deionized water.

2. Heat to boiling to fully dissolve.

3. Dispense into suitable containers.

4. Sterilize by autoclaving at 121℃ for 15 minutes.

5. Mix well after sterilization and store until use.