SSM Broth – 250g | Buffered Enrichment Medium for Bacterial Cultivation

SSM Broth is a phosphate-buffered liquid medium optimized for general bacterial cultivation, providing stable pH conditions for reliable growth in diverse applications from environmental sampling to industrial bioprocessing.

Key Applications:

✔️ Enrichment of environmental bacteria in water/soil samples

✔️ Wastewater treatment efficacy monitoring

✔️ Recovery of stressed microorganisms

✔️ Bacterial growth studies requiring pH stability

Scientifically Formulated (Per Liter):

| Component | Quantity |

|————————-|———-|

| Na₂HPO₄ | 6.0 g |

| KH₂PO₄ | 3.0 g |

| MgSO₄·7H₂O | 0.2 g |

| Glucose | 5.0 g |

| Casein Hydrolysate | 20.0 g |

Self-buffering at neutral pH

Simplified Protocol:

1️⃣ Dissolve 34.2g powder in 1L distilled water

2️⃣ Sterilize by autoclaving at 121°C for 15 minutes

3️⃣ Cool to 25-30°C before inoculation

Critical Advantages:

– Dual-phosphate buffer system: Maintains pH stability during extended incubation

– High nutrient load: 20g/L casein hydrolysate supports fastidious organisms

– Magnesium sulfate: Enhances enzyme activity for improved growth

– Ready-to-use powder: Ensures batch-to-batch consistency

– 3-year shelf life: Ambient storage without performance loss

Technical Validation:

– Optimized for turbidimetric growth measurement

– Compatible with most aerobic bacterial species