SSM Broth – 250g | Buffered Enrichment Medium for Bacterial Cultivation
SSM Broth is a phosphate-buffered liquid medium optimized for general bacterial cultivation, providing stable pH conditions for reliable growth in diverse applications from environmental sampling to industrial bioprocessing.
Key Applications:
✔️ Enrichment of environmental bacteria in water/soil samples
✔️ Wastewater treatment efficacy monitoring
✔️ Recovery of stressed microorganisms
✔️ Bacterial growth studies requiring pH stability
Scientifically Formulated (Per Liter):
| Component | Quantity |
|————————-|———-|
| Na₂HPO₄ | 6.0 g |
| KH₂PO₄ | 3.0 g |
| MgSO₄·7H₂O | 0.2 g |
| Glucose | 5.0 g |
| Casein Hydrolysate | 20.0 g |
Self-buffering at neutral pH
Simplified Protocol:
1️⃣ Dissolve 34.2g powder in 1L distilled water
2️⃣ Sterilize by autoclaving at 121°C for 15 minutes
3️⃣ Cool to 25-30°C before inoculation
Critical Advantages:
– Dual-phosphate buffer system: Maintains pH stability during extended incubation
– High nutrient load: 20g/L casein hydrolysate supports fastidious organisms
– Magnesium sulfate: Enhances enzyme activity for improved growth
– Ready-to-use powder: Ensures batch-to-batch consistency
– 3-year shelf life: Ambient storage without performance loss
Technical Validation:
– Optimized for turbidimetric growth measurement
– Compatible with most aerobic bacterial species
