D/E Neutralizing Broth Base – 250g | Cosmetic Microbiological Testing

Complete Disinfectant Neutralization for Cosmetic Microbiology

D/E Neutralizing Broth Base is an essential medium for accurate microbial testing of cosmetic products. Its unique formulation inactivates common preservatives and disinfectants, enabling true recovery of microorganisms in personal care samples.

Critical Applications:

– Preservative efficacy testing (PET)

– Cosmetic product microbial limits testing

– Disinfectant residue neutralization

– Sanitizer validation studies

– Topical product contamination recovery

Key Advantages:

✓ Broad-Spectrum Neutralization – Inactivates quats, phenolics, chlorine

✓ Growth Promotion – Lecithin/Tween system supports stressed cells

✓ Visual pH Indicator – Bromcresol purple detects acid production

✓ Ready-to-Modify – Pre-measured Tween 80 addition protocol

✓ USP/ISO Compliant – Meets cosmetic microbiology standards

Formula Composition (per liter):

– Pancreatic Digest of Casein: 5.0g

– Glucose: 10.0g

– Soybean Lecithin: 7.0g

– Sodium Thiosulfate: 6.0g

– Sodium Hydrogensulfite: 2.5g

– Yeast Extract: 2.5g

– Sodium Thioglycollate: 1.0g

– Bromcresol Purple: 0.02g

– Final pH: 7.6 ± 0.2 (25°C)

Preparation Protocol:

1. Suspend 34.0g in 1L distilled water

2. Heat to 80°C with vigorous stirring

3. Add 1g Tween 80 per 200ml broth

4. Autoclave at 121°C for 15 minutes

Note: Clumping during suspension is normal – continue heating to dissolve

Quality Validation:

– Neutralization capacity tested against benzalkonium chloride

– Growth recovery ≥70% with Pseudomonas aeruginosa

– Low background bioburden

Store sealed below 30°C. Shelf life: 18 months.