Ziehl-Neelsen Stain for Acid-Fast Bacteria Detection
Ziehl-Neelsen Stain (Acid-Fast Stain) is a specialized laboratory reagent kit designed for the detection and differentiation of acid-fast bacteria through microscopic examination. Its precise formulation ensures clear visualization of target bacteria, making it an essential tool for microbial research and analysis.
Key Specifications:
Packaging: 250ml/bottle × 3 bottles/set
Storage Condition: 2-8°C, protected from light
Shelf Life: 1 year
Formulation:
Primary Stain: 0.3g basic fuchsin, 10ml 95% alcohol, 90ml 5% carbolic acid
Decolorizing Solution: 3% hydrochloric acid in alcohol
Counterstain: 0.3g methylene blue, 30ml 95% alcohol, 100ml 0.01% potassium hydroxide
Intended Use:
Specifically formulated for identifying acid-fast bacteria, enabling clear differentiation from other bacteria and cells under microscopy.
How to Use:
Prepare a bacterial smear.
Fix the smear by passing through a flame.
Apply the primary stain, gently heat until steam appears, then remove from heat. Stain for 5 minutes (ensure the sample remains covered with stain by adding more if needed; avoid drying). After 5 minutes, rinse gently with distilled or deionized water and blot excess moisture with absorbent paper.
Apply the decolorizing solution repeatedly for 1-3 minutes until no visible red color remains. Rinse with water and blot dry.
Apply the counterstain and stain for 30-60 seconds. Rinse with water and blot excess moisture.
Microscopic examination: Acid-fast bacteria appear red against a light blue background; other bacteria and cells appear blue.