Key Features & Benefits:
- Standard Yeast Medium: The most widely used rich medium for general yeast culture in research laboratories.
- Optimized for Molecular Biology: Supports healthy growth of yeast strains used in transformation, plasmid maintenance, and gene expression studies.
- Rich Formulation: Balanced mixture of peptone and yeast extract provides essential growth factors for vigorous yeast propagation.
- Versatile Use: Ideal for routine subculturing, short-term storage on slants, and as a base for selective media when supplemented.
Product Specifications
| Specification | Details |
|---|---|
| Product Type | Dehydrated Culture Media |
| Quantity | 250g (Prepares approximately 3.85 Liters of medium) |
| Application | Yeast culture and maintenance; Molecular biology research |
| Storage | Room temperature (Stable for 3 years) |
| Organisms | Saccharomyces cerevisiae and other yeasts |
Composition (per liter)
| Component | Amount |
|---|---|
| Peptone | 20.0g |
| Yeast Extract | 10.0g |
| Dextrose (Glucose) | 20.0g |
| Agar | 15.0g |
Final pH: 6.5 ± 0.2 at 25°C
Instructions for Preparation
- Suspend 65.0g of the powder in 1 liter of distilled or deionized water.
- Heat and boil to dissolve the medium completely.
- Dispense into final containers.
- Sterilize by autoclaving at 121°C for 15 minutes.
- After sterilization, gently swirl the vessel to prevent agar sedimentation.
- Cool and pour into plates or prepare slants as required.