Yeast Mannitol Broth (YMB) – Specialized Medium for Rhizobium & Agrobacterium Culture (500g)
Defined Yeast Mannitol Broth for Soil Microorganism Isolation and Enumeration
Specifically formulated for rhizosphere bacteria, this medium combines 10.0g/L mannitol as carbon source with 1.0g/L yeast extract to promote optimal growth of Rhizobium, Agrobacterium, and other nitrogen-fixing bacteria. The inclusion of 1.0g/L CaCO₃ maintains pH stability at 6.8±0.2, simulating natural soil conditions for ecological studies.
Critical Applications:
🌱 Soil Microbiology: Isolation and enumeration of Rhizobium/Agrobacterium
🔬 Nitrogen Fixation Studies: Research on symbiotic plant-microbe interactions
🌾 Agricultural Research: Biofertilizer development and strain screening
Technical Advantages:
✅ Specialized Carbon Source: 10.0g/L mannitol for rhizobial metabolism
✅ pH Buffering: 1.0g/L CaCO₃ maintains 6.8±0.2
✅ Mineral Balance: 0.5g/L K₂HPO₄ + 0.2g/L MgSO₄ mimics soil chemistry
✅ Low-Salt Formula: 0.1g/L NaCl prevents osmotic shock
Preparation Protocol:
Suspend 12.8g in 1L purified water
Stir continuously during dispensing into vessels
Autoclave at 121°C for 15 minutes
Complete Formulation (Per Liter):
Yeast Extract: 1.0g
Mannitol: 10.0g
K₂HPO₄: 0.5g
MgSO₄: 0.2g
NaCl: 0.1g
CaCO₃: 1.0g
