VRE Broth Base – Enrichment Medium for Vancomycin-Resistant Enterococci (VRE) and High-Level Aminoglycoside-Resistant Enterococci (HLARE), 250g

VRE Broth Base is a selective enrichment medium formulated for the isolation of vancomycin-resistant enterococci (VRE) and high-level aminoglycoside-resistant enterococci (HLARE) from clinical and environmental samples. Supplementation with 2 mg/L meropenem enhances selectivity by inhibiting non-target bacteria.

Key Features:

• Designed for VRE and HLARE isolation

• Requires supplementation with 2 mg/L meropenem

• Reliable for clinical diagnostics and antimicrobial resistance studies

• Supplied in 250 g bottle, with long shelf life

Composition (per liter):

• Calf brain infusion solids …………….. 12.5 g

• Beef heart infusion solids …………….. 5.0 g

• Proteose Peptone …………………….. 10.0 g

• Glucose ……………………………… 2.0 g

• Sodium Chloride ………………………. 5.0 g

• Disodium Phosphate …………………… 2.5 g

• Final pH: 7.1 ± 0.2 at 25℃

Preparation Instructions:

1. Suspend 38.0 g of VRE Broth Base in 1 liter of distilled or deionized water.

2. Heat with frequent agitation until completely dissolved.

3. Dispense into suitable containers.

4. Sterilize by autoclaving at 121℃ for 15 minutes.

5. Cool to about 50℃.

6. Under aseptic conditions, add 2 mg sterile meropenem per liter.

7. Mix thoroughly and store until use.

Storage:

• Store at room temperature in a dry place.

• Shelf life: 3 years.