Vitamin Free Yeast Base | Yeast Classification Medium

Product Name: Vitamin Free Yeast Base
Application: Used for classification of yeast strains based on vitamin utilization.
Packing Size: 250 g/bottle
Storage: Room temperature
Shelf Life: 3 years

Formula (per liter):

• Ammonium Sulfate – 5000 mg

• Glucose – 10000 mg

• DL-Tryptophan – 20 mg

• L-Histidine Hydrochloride – 10 mg

• DL-Methionine – 20 mg

• Calcium Chloride – 100 mg

• Boric Acid – 50.0 mg

• Copper Sulfate – 0.04 mg

• Potassium Iodide – 0.1 mg

• Ferric Chloride – 0.2 mg

• Manganese Sulfate – 0.4 mg

• Sodium Molybdate – 0.2 mg

• Zinc Sulfate – 0.4 mg

• Magnesium Sulfate – 500 mg

• Potassium Dihydrogen Phosphate – 1000 mg

• Sodium Chloride – 100 mg

• Final pH: 5.6 ± 0.2 (at 25°C)

Preparation Instructions:
Dissolve 16.8 g of the medium in 100 mL of distilled or deionized water. Add selected vitamins if required. Warm slightly to dissolve completely. Sterilize by filtration. Prepare as a 10× concentrated stock solution and store at 2–8°C.

For use, transfer 0.5 mL of the stock solution aseptically into 4.5 mL of sterile distilled water.