Key Features
- Designed for yeast classification studies
- Supports evaluation of vitamin utilization by yeast
- Suitable for microbiology research laboratories
- Dehydrated powder formulation for easy preparation
- Stable composition and reliable experimental performance
Applications
- Yeast classification and taxonomy
- Vitamin utilization studies in yeast
- Microbial physiology research
- Microbiology laboratory testing
Product Specifications
- Product Name: Vitamin Free Yeast Base
- Form: Dehydrated powder
- Package Size: 250 g / bottle
- Storage Condition: Room temperature
- Shelf Life: 3 years
- Final pH (25°C): 5.6 ± 0.2
Composition (per liter)
| Component | Amount |
|---|---|
| Ammonium Sulfate | 5000 mg |
| Dextrose | 10000 mg |
| DL-Tryptophan | 20 mg |
| L-Histidine Hydrochloride | 10 mg |
| DL-Methionine | 20 mg |
| Calcium Chloride | 100 mg |
| Boric Acid | 50.0 mg |
| Copper Sulfate | 0.04 mg |
| Potassium Iodide | 0.1 mg |
| Ferric Chloride | 0.2 mg |
| Manganese Sulfate | 0.4 mg |
| Sodium Molybdate | 0.2 mg |
| Zinc Sulfate | 0.4 mg |
| Magnesium Sulfate | 500 mg |
| Monopotassium Phosphate | 1000 mg |
| Sodium Chloride | 100 mg |
Preparation Instructions
- Dissolve 16.8 g of the medium in 100 mL of distilled or deionized water.
- Selectively add the required vitamins as needed.
- Warm gently to dissolve completely.
- Sterilize by membrane filtration to prepare a 10× concentrated solution.
- Store the concentrated solution at 2–8°C.
Working Solution Preparation
- Under aseptic conditions, transfer 0.5 mL of the prepared concentrate.
- Add to 4.5 mL of sterile distilled water using a sterile pipette.
- Mix well before use.