This Vibrio parahaemolyticus Biochemical Identification Kit is a comprehensive 10-test system designed for the biochemical confirmation of Vibrio parahaemolyticus. The kit is configured according to the GB/T 4789.7 standard for the microbiological examination of food and water samples.
The kit provides the necessary biochemical media for presumptive identification and differentiation of V. parahaemolyticus from related Vibrio species. It includes tests for halophilism (salt tolerance), amino acid decarboxylases (lysine), carbohydrate fermentation (mannitol), Voges-Proskauer (V-P) reaction, and ONPG (β-galactosidase) activity.
Each kit contains sufficient materials for 10 complete sets (10 tests × 2 sets/box × 5 boxes). The media are supplied in sealed ampoules for convenience and safety. Sterile liquid paraffin is included for overlaying the decarboxylase broths. Oxidase test paper is also provided.
Important Notes for Purchase:
- V-P Reagent Kit (HB8281): Required for the V-P test but sold separately.
- 3% NaCl Triple Sugar Iron (TSI) Agar: Required for the initial screening but not included in this kit.
Intended Use
- Biochemical identification and confirmation of Vibrio parahaemolyticus.
- Microbiological testing of seafood, shellfish, and marine products.
- Food safety and hygiene verification according to GB/T 4789.7.
- Quality control in food and pharmaceutical laboratories.
- Research and educational laboratory applications.
Kit Contents (10 Tests, 2 Sets per Box, 5 Boxes)
- Salt-Free Tryptone Water: 2 ampoules
- 6% NaCl Tryptone Water: 2 ampoules
- 8% NaCl Tryptone Water: 2 ampoules
- 10% NaCl Tryptone Water: 2 ampoules
- 3% NaCl V-P Semi-Solid Medium: 2 ampoules
- 3% NaCl Mannitol: 2 ampoules
- 3% NaCl Lysine Decarboxylase Broth: 2 ampoules
- 3% NaCl Amino Acid Decarboxylase Control: 2 ampoules
- 3% NaCl ONPG: 2 ampoules
- Oxidase Test Paper: 1 pack
- Sterile Liquid Paraffin: 1 ampoule
- Sealing Film: Included
Results Interpretation (Reference)
| Test | Positive | Negative | Incubation |
|---|---|---|---|
| Oxidase | Blue / blue-purple | No color change | — |
| Lysine Decarboxylase | Purple | Yellow | 24–48 h |
| Decarboxylase Control | Yellow | Yellow | 24–48 h |
| Salt-Free Tryptone | Turbid growth | No / weak growth | 24 h |
| 6% NaCl Tryptone | Turbid growth | No / weak growth | 24 h |
| 8% NaCl Tryptone | Turbid growth | No / weak growth | 24 h |
| 10% NaCl Tryptone | Turbid growth | No / weak growth | 24 h |
| Mannitol | Yellow | Blue-green / no change | 24–48 h |
| V-P (Motility) | Growth spreads from stab line | Clear stab line | 24–48 h |
| V-P (Reaction) | Red | No color change | 24–48 h |
| ONPG | Yellow | Colorless / no change | 24 h |
Note: For decarboxylase tests, add sterile liquid paraffin overlay after inoculation. For V-P test, add V-P reagent (0.3 mL α-naphthol, 0.1 mL KOH) after incubation and observe within 4 hours.
Storage and Shelf Life
- Format: Ampoules in a multi-box kit (10 tests × 2 sets/box × 5 boxes).
- Shelf Life: 1 year from date of manufacture.
- Storage: Store at 2–8°C. Do not freeze.
Q: What is this kit used for?
A: This kit is used for the biochemical identification and confirmation of Vibrio parahaemolyticus in food and environmental samples according to the GB/T 4789.7 standard.
Q: What is included in the kit?
A: The kit includes 10 different biochemical tests (halophilism, decarboxylase, mannitol, V-P, ONPG), plus oxidase test paper, sterile liquid paraffin, and sealing film. It contains enough material for 10 complete sets.
Q: Are all required reagents included?
A: No. The V-P Reagent Kit (HB8281) is required for the V-P test but is sold separately. The 3% NaCl TSI Agar is also not included and must be prepared or purchased separately.
Q: How do I interpret the halophilism tests?
A: Growth in salt-free, 6%, 8%, and 10% NaCl tryptone water indicates the salt tolerance profile of the organism. V. parahaemolyticus typically grows in 6–8% NaCl but not in 10% NaCl or salt-free medium.
Q: How do I perform the V-P test?
A: After incubation, add V-P reagent (0.3 mL α-naphthol, 0.1 mL KOH) to the V-P semi-solid medium. A red color within 4 hours indicates a positive result.
Q: Is this kit intended for clinical use?
A: No. This kit is intended for laboratory quality control and food safety testing only. It is not intended for clinical diagnosis.
