Urease Test Base Medium with Urea Supplement
Complete System for Urease Activity Detection
This base medium requires post-sterilization addition of urea to accurately identify urease-producing bacteria through distinctive phenol red color transition.
⚠️ Critical Preparation:
△ Must add 20% sterile urea solution (4g/100ml)
△ Add at 50-55°C to prevent urea degradation
△ For trained microbiologists only
Key Features:
Optimized phosphate buffer (pH 6.9±0.1)
Low peptone concentration prevents false positives
Room temperature stable (3-year shelf life)
Complies with CLSI M35-A standards
Technical Specifications:
▶ Base Formula (per liter):
Peptone 1.0g | K₂HPO₄ 2.0g
NaCl 5.0g | Dextrose 1.0g | Phenol Red 0.012g
▶ pH: 6.9±0.1 (25°C)
▶ Preparation:
9g/L → sterilize at 121°C/15min
Cool 50-55°C → add 40g/L urea
Diagnostic Color Transition:
Positive: Deep pink/red (alkaline)
Negative: Yellow/orange (acidic)
Applications:
Identification of Proteus and Helicobacter
Differentiation of Enterobacteriaceae
Urinary pathogen screening
