Tryptone Yeast Cystine (TYC) Broth Medium, Selective Medium for Streptococcus Isolation, 250g

Tryptone Yeast Cystine (TYC) Broth Medium is designed for the isolation, cultivation, and enrichment of blood streptococci from clinical, oral, and environmental samples.
The medium contains tryptone and yeast extract as rich sources of nitrogen and growth factors, L-cystine to support streptococcal growth, and sucrose for differentiation and metabolic activity. Sodium acetate and other buffering salts help maintain pH stability.

When supplemented with Bacitracin solution (final concentration: 0.2 U/ml), TYC Broth exhibits enhanced selectivity by inhibiting background flora, making it suitable for the detection and study of streptococci in complex microbiota.

Key Features:

• Formulated for blood streptococci cultivation and isolation

• Supports streptococci growth with rich peptone, yeast extract, and cystine

• Optional Bacitracin supplement enhances selectivity

• Pack size: 250g/bottle

• Shelf life: 3 years

📌 Composition (per liter)

• Tryptone ……………………………. 15.0 g

• Yeast Extract ………………………… 5.0 g

• L-Cystine ……………………………. 0.2 g

• Sodium Sulphite ………………………. 0.1 g

• Sodium Chloride ………………………. 1.0 g

• Disodium Phosphate Anhydrous …………… 0.8 g

• Sodium Bicarbonate …………………… 2.0 g

• Sodium Acetate Anhydrous ……………… 12.0 g

• Sucrose ……………………………… 50.0 g

• Final pH: 7.3 ± 0.2 (25℃)

📌 Instructions for Use

1. Suspend 86.0 g of the medium in 1 liter of distilled or deionized water (adjust proportionally as needed).

2. Heat to boiling until completely dissolved.

3. Dispense into suitable containers.

4. Sterilize by autoclaving at 121℃ for 15 minutes.

5. Cool to room temperature for use.