Trypticase Soy Broth (TSB) – Universal Enrichment Medium for Bacterial Culture (250g)
Industry-Standard Trypticase Soy Broth for Robust Microbial Growth
This versatile enrichment broth combines pancreatic digest (15.0g/L tryptone) and enzymatic soybean digest (5.0g/L) to create optimal conditions for cultivating diverse bacterial species. With balanced electrolytes (5.0g/L NaCl) and pH-stabilized at 7.2±0.2, it’s the preferred medium for clinical diagnostics, pharmaceutical QC, and industrial bioprocessing.
Key Applications:
🧫 Clinical Enrichment: Blood cultures and sterile fluid testing
🏭 Fermentation Seed: Prepares high-density inoculum
🧪 Environmental Testing: Water and surface sampling
💊 Pharmaceutical QC: USP-compliant sterility testing
Technical Advantages:
✅ Dual Protein System: 15.0g/L tryptone + 5.0g/L soybean digest
✅ Osmotic Balance: 5.0g/L NaCl maintains cell integrity
✅ pH-Optimized: 7.2±0.2 for consistent growth kinetics
✅ Ready-to-Use: 25.0g/L standardized concentration
Preparation Protocol:
Suspend 25.0g in 1L purified water
Warm to 45-50°C for complete dissolution
Dispense into tubes or flasks
Autoclave at 121°C for 15 minutes
Complete Formulation (Per Liter):
Tryptone: 15.0g
Enzymatic Soybean Digest: 5.0g
NaCl: 5.0g
