Sealed bottle of Trypticase Soy Broth powder

Trypticase Soy Broth (TSB) | General Purpose Microbial Broth

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Trypticase Soy Broth (TSB) is a general-purpose, nutrient-rich liquid medium designed for the cultivation and enrichment of a wide range of bacteria.
It is commonly used in microbiology laboratories for routine bacterial growth, inoculum preparation, and enrichment procedures.

The balanced formulation based on casein and soybean digests provides essential nutrients required for consistent bacterial growth.
This dehydrated medium is suitable for use in pharmaceutical, food, environmental, and industrial microbiology laboratories.


🔬 Product Applications

  • Bacterial enrichment and cultivation

  • General microbiological testing

  • Inoculum preparation

  • Quality control and routine laboratory use

  • Pharmaceutical, food, and environmental microbiology


📦 Product Specifications

  • Product Name: Trypticase Soy Broth (TSB)

  • English Name: Trypticase Soy Broth

  • Form: Dehydrated powder

  • Package Size: 250 g / bottle

  • Storage Condition: Room temperature, dry and sealed

  • Shelf Life: 3 years


🧾 Medium Composition (per liter)

  • Tryptone – 15.0 g

  • Enzymatic Digest of Soybean Meal – 5.0 g

  • Sodium Chloride – 5.0 g

Final pH: 7.2 ± 0.2 (25 °C)


⚙️ Directions for Use

  1. Suspend 25.0 g of the medium in 1 liter of distilled or deionized water.

  2. Warm gently while stirring until completely dissolved.

  3. Dispense into tubes or flasks as required.

  4. Sterilize by autoclaving at 121 °C for 15 minutes.

  5. Cool and use as needed.

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