Tryptic Soy Broth (TSB) – 250g | Multipurpose Enrichment Broth

Versatile Enrichment Broth for Demanding Bacteriology

Tryptic Soy Broth (TSB) delivers balanced nutrition for robust growth of diverse microorganisms. With high peptone content and energy sources, it serves as an ideal base for enrichment cultures, MPN assays, and antibiotic-modified media preparation.

Key Applications:

– Broad-spectrum bacterial enrichment

– Staphylococcus aureus MPN determination

– Seed cultures for fermentation

– Diagnostic media foundation

– Environmental isolate amplification

Critical Advantages:

✓ Superior Growth Yield – 17g/L tryptone supports fast proliferation

✓ MPN-Compliant – Validated for S. aureus most probable number tests

✓ Customizable Base – Ready for antibiotic supplements (e.g. mTSB)

✓ Rapid Solubility – Dissolves in ≤3 minutes at 45°C

Formula Composition (per liter):

– Tryptone: 17.0g

– Enzymatic Digest of Soybean Meal: 3.0g

– Dipotassium Phosphate: 2.5g

– Sodium Chloride: 5.0g

– Dextrose: 2.5g

– Final pH: 7.3 ± 0.2 (25°C)

Preparation Protocol:

Standard TSB:

1. Suspend 30.0g in 1L distilled water

2. Warm to 45°C with stirring until dissolved

3. Autoclave at 121°C for 15 minutes

Double-Strength TSB:

– Use 60.0g per liter

Modified TSB (mTSB) Preparation:

1. Prepare and sterilize base broth

2. Cool to ≤50°C

3. Aseptically add per 225ml:

– Polymyxin B (2.25mg)

– Nalidixic Acid (2.25mg)

Quality Certification:

– Growth promotion tested with Staphylococcus aureus ATCC 6538

– USP-grade raw materials

– Endotoxin ≤5 EU/mL

Store sealed at 10-30°C. Shelf life: 36 months.