Triple Sugar Iron Agar Medium (TSI Agar) | 250g Powder

Product Name

Triple Sugar Iron Agar Medium

Specification

250 g / bottle

Shelf Life

3 years

Storage

Room temperature

Product Overview

Triple Sugar Iron Agar Medium (TSI Agar) is a dehydrated microbiological culture medium designed for biochemical differentiation of Enterobacteriaceae. The medium is widely used in microbiology laboratories for carbohydrate utilization and hydrogen sulfide production testing.

This formulation complies with pharmacopoeial requirements and is suitable for use in pharmaceutical microbiology, clinical laboratories, food testing, and research applications.

Application

• Biochemical testing of Enterobacteriaceae

• Differentiation of gram-negative intestinal bacteria

• Routine microbiological identification procedures

Reference Standards

• European Pharmacopoeia (EP)

• United States Pharmacopeia (USP)

Medium Composition (per liter)

• Pancreatic Digest of Casein: 10.0 g

• Peptic Digest of Animal Tissue: 10.0 g

• Sodium Chloride: 5.0 g

• Lactose: 10.0 g

• Sucrose: 10.0 g

• Dextrose: 1.0 g

• Ferric Ammonium Citrate: 0.2 g

• Sodium Thiosulfate: 0.2 g

• Phenol Red: 0.025 g

• Agar: 13.0 g

Final pH: 7.3 ± 0.2 (at 25 °C)

Preparation Instructions

Suspend 59.4 g of the medium in 1 liter of distilled or deionized water. Heat with agitation and boil until completely dissolved. Dispense into test tubes and sterilize by autoclaving at 121 °C for 15 minutes. After sterilization, allow the medium to solidify in a deep slant position before use.

Handling & Storage

• Store the dehydrated medium in a tightly sealed container

• Keep in a dry place at room temperature

• For laboratory use only