Trehale-Proline Medium for Actinomycete Isolation and Cultivation – 250g

Trehale-Proline Medium is a selective culture medium designed for the isolation and cultivation of Actinomycetes. The formulation contains trehalose and proline as key nutrient sources, supporting actinomycete growth while inhibiting unwanted microbial contaminants. It is commonly used in microbiological laboratories for strain isolation and research.

Product Formulation (per liter):

• Trehalose: provided as main carbon source

• Proline: provided as nitrogen source

• Additional balanced nutrients for selective cultivation

Instructions for Use:

1. Dissolve 27.0g of the medium in 1L of distilled or deionized water.

2. Heat and stir until boiling for at least 1 minute.

3. Dispense into appropriate containers.

4. Sterilize at 116°C for 30 minutes.

5. After sterilization, shake gently before use as the medium contains insoluble particles.

Packaging & Storage:

• 250g/bottle

• Store at room temperature

• Shelf life: 3 years