Todd-Hewitt Broth – Streptococci Culture and Beta-Hemolysin Preparation Medium, 250g

Todd-Hewitt Broth is widely used in clinical and research microbiology for cultivating streptococci and preparing beta-hemolysin. Its balanced nutrient composition promotes rapid growth while maintaining the desired physiological properties of the bacteria.

Composition (per liter):

• Peptone – 20.0 g

• Heart Extract Powder – 3.1 g

• Dextrose – 2.0 g

• Sodium Chloride – 2.0 g

• Disodium Hydrogen Phosphate – 0.4 g

• Sodium Carbonate – 2.5 g

• Final pH: 7.8 ± 0.1 (at 25℃)

Preparation Instructions:

1. Dissolve 30.0 g of dehydrated medium in 1 liter of distilled or deionized water (adjust proportionally for other volumes).

2. Heat to boiling until completely dissolved.

3. Dispense into suitable containers.

4. Sterilize by autoclaving at 121℃ for 15 minutes.

5. Cool and store under aseptic conditions until use.

Storage:
Store in a tightly sealed container at room temperature in a dry place. Shelf life: 3 years.

Applications:

• Cultivation of streptococci

• Preparation of beta-hemolysin for research or testing

• Clinical and laboratory microbiology studies