Thioglycollate Broth Medium – Versatile Fluid Medium for Aerobic/Anaerobic Cultures (250g)
USP-Compliant Thioglycollate Broth for Broad-Spectrum Microbial Cultivation
This multipurpose fluid medium contains sodium thioglycollate (0.5g/L) and L-cystine (0.5g/L) to create oxygen gradients suitable for both aerobic and anaerobic bacteria cultivation. Ideal for sterility testing, blood cultures, and fastidious organism recovery.
Key Applications:
🧫 Sterility Testing: USP/EP compliant medium for pharmaceutical QC
🩸 Blood Cultures: Detects bacteremia/fungemia in clinical labs
🦠 Anaerobic Studies: Supports growth of oxygen-sensitive organisms
Technical Advantages:
✅ Redox Balance: 0.5g/L thioglycollate maintains anaerobic conditions
✅ Nutrient-Rich: 15.0g/L casein peptone + 5.0g/L yeast extract
✅ Energy Supply: 5.5g/L dextrose enhances fastidious growth
✅ Ready-to-Use: 29.0g/L standardized concentration
Preparation Protocol:
Suspend 29.0g in 1L purified water
Boil until complete dissolution
Autoclave at 121°C/15min or 115°C/30min
Critical: Rapid cooling after sterilization
Complete Formulation (Per Liter):
Pancreatic Casein Digest: 15.0g
Yeast Extract: 5.0g
L-Cystine: 0.5g
Sodium Thioglycollate: 0.5g
NaCl: 2.5g
Dextrose: 5.5g
