Standard II Nutrient Agar – 250g | pH-Indicator Bacterial Culture

Visual pH Monitoring for Bacterial Metabolism Studies

Standard II Nutrient Agar with bromcresol purple (0.04g/L) enables real-time detection of acid production during microbial growth. The elevated glucose content (5g/L) enhances fermentation reactions.

Key Applications:

– Bacterial fermentation capability screening

– Carbohydrate utilization testing

– Dairy starter culture evaluation

– Acid-producing pathogen identification

– Educational microbiology demonstrations

Technical Specifications:

– Formula (per liter):

– Tryptone: 10.0g

– Glucose: 5.0g

– Bromcresol Purple: 0.04g

– Agar: 15.0g

– pH: 6.9 ± 0.1 (25°C)

– Packaging: 250g/bottle

– Storage: Room temperature (15-25°C)

– Shelf Life: 3 years

Preparation Protocol:

1. Suspend 30.0g in 1L distilled water

2. Boil with continuous stirring until dissolved

3. Autoclave at 121°C for 15 minutes

4. Swirl during cooling for even indicator distribution

Visual Indicators:

– Purple: pH >6.8 (neutral/alkaline)

– Yellow: pH <5.2 (acidic)