Standard I Nutrient Broth – 250g | High-Yield Bacterial Culture

Enhanced Nutrient Broth for Demanding Bacterial Cultures

Standard I Nutrient Broth provides superior growth conditions with elevated peptone content (15g/L) and balanced electrolytes. Ideal for applications requiring rapid biomass accumulation.

Key Applications:

– Seed culture preparation for fermentation

– Antibiotic susceptibility testing

– Diagnostic specimen enrichment

– Environmental water sample analysis

– Vaccine production seed stocks

Technical Specifications:

– Formula (per liter):

– Peptone: 15.0g

– Yeast Extract: 3.0g

– Sodium Chloride: 6.0g

– D(+)Glucose: 1.0g

– pH: 7.5 ± 0.2 (25°C)

– Packaging: 250g/bottle

– Storage: Room temperature (15-25°C)

– Shelf Life: 3 years

Preparation Protocol:

1. Suspend 25.0g in 1L distilled water

2. Dissolve at 45-50°C with stirring (≤3 minutes)

3. Autoclave at 121°C for 15 minutes

Quality Advantages:

– 2X faster doubling time vs standard formulations

– Optical clarity for turbidity measurements

– Low particulate formulation